Microfluidic Culture Chamber for the Long-term Perfu- Sion and Precise Chemical Stimulation of Organotypic Brain Tissue Slices
نویسندگان
چکیده
We have developed a microfluidic perfusion-based culture system to study long-term in-vitro responses of organotypic brain slices exposed to localized neurochemical stimulation. Using this microperfusion chamber we show that hippocampal organotypic brain slices cultures grown on nitrocellulose membranes can be stimulated for up to 24 hours in our experimental setup while preserving tissue viability. By using fluorescent probe we show that a specific area of the hippocampal slice can be precisely targeted and stimulated. The device allows a virtual pixelisation of slice, precise control of the in-vitro micro environment, long-term culture of viable brain slices, and delivery of fluids to selected brain regions in a multiplexed and spatially defined manner.
منابع مشابه
Design and Fabrication of a Pdms Microfluidic Chamber for Microfluidic Experiments with Organotypic Brain Slices
1 Biomedical Engineering Department, McGill University, CANADA 2 Department of Pharmacology and Therapeutics, McGill University, CANADA Email: [email protected] ABSTRACT Microfluidic systems are increasingly being used for the culture and study of dissociated cells because they facilitate drug screening and chemotaxis studies using minute amounts of chemicals. However, microfluidics ...
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